Protein assay was developed by Bradford in 1976. It is a quick and correct procedure used to measure the total concentration of protein in a given sample. The reaction depends on the composition of amino acids in the proteins measured by the procedure. Its principle is that the protein is bound with Coosmassie dye which results in color change under acidic conditions.
This dye comes in three different forms named as anionic which is blue in color, neutral which is green, and cationic which is red in color. Under acidic conditions, the red color of the dye is changed into blue form due to the binding of the protein being assayed. If there's no protein present for binding, then the solution will remain brown in color. Many protein assay kits are available which are ready-to-use, detergent compatible in nature.
A variety of protein assay kits are available for the reliable measurement of total protein concentration present in the given solution. One of the most common protein assay kits is the bicinchoninic acid assay or BCA assay kit which utilizes copper reduction in an alkaline solution gives purple color due to the presence of the bicinchoninic acid. This colorimetric change from green to purple is measured by a spectrometer.
The Micro BCA Protein Kit is a detergentcompatible bicinchoninic acid kit that is used for the same purpose as a BCA protein assay kit is used i.e. for the colorimetric detection and quantitation of total protein present in the given sample. This kit has been optimized to use with dilute protein samples.
Commercial BCA protein assay kits are used to perform such methods while providing tested quality and ready-to-use reagents for both the speed and reproductivity of experiments. You need to keep an eye on the buffer of components of the given sample to ensure its chemical compatibility with the assay.